York Valkyrie will launch their defence of their Women's Super League title against Wigan Warriors on Friday May 16 at The Brick Community Stadium, live on Sky Sports.
The Valkyrie made history by becoming the first team to retain their crown as they denied St Helens the treble in October in front of a record crowd, with Lindsay Anfield's side now on the hunt for a third successive title.
Saints will be looking to bounce back from that agonising defeat under new head coaches Craig Richards and Derek Hardman, with new signings Amy Hardcastle, Shona Hoyle and Caitlin Casey all set to face their former club in a Challenge Cup Final rematch against Leeds Rhinos on May 17 at the Totally Wicked Stadium, with kick-off at 2pm.
Sky Sports will showcase three fixtures once again this year, in addition to the play-offs and Grand Final.
Leigh Leopards secured a place in the top flight for 2025 after defeating Featherstone Rovers 34-16 in the promotion play-off final and will begin life in the Super League on May 18 at home to Amanda Wilkinson's Barrow Raiders.
On the same day, Warrington Wolves host Huddersfield Giants at the Halliwell Jones Stadium, with both sides hoping that 2025 could be the year they make ground on the top four teams in the competition.
The second live game of the season will be on Friday July 11 when Leeds take on Saints at AMT Headingley at 5.30pm - the first part of a double header which also features the men's teams in Round 18 of the Super League.
Just a few weeks later, Wigan will travel to the Totally Wicked Stadium on September 5 to face the Saints, also live on Sky Sports, where they will be looking to avenge their 2024 semi-final defeat.
The 2025 campaign will have 14 rounds, concluding on the weekend of September 14/15, before the top four face off in the play-offs on September 21 for a spot in this year's Grand Final on Sunday October 5 - which will be hosted at the home ground of the highest-ranked club.
All eight sides will begin their hunt for silverware in the Betfred Challenge Cup on the weekend of April 5/6, with the final returning to Wembley Stadium for only the third time on Saturday June 7.
Thomas Brindle, general manager of the Betfred Women's Super League, said: "2025 is shaping up to be an incredibly exciting year for women's rugby league, which kicks off in March with a thrilling clash between England and the Australian Jillaroos in Las Vegas.
"Without a doubt, there will be some emerging young talent on display continuing to make their mark on the game alongside the senior internationals - I wish them all the best for that fixture.
"This upcoming season promises some high-intensity, thrilling match-ups from the very beginning, including St Helens looking for redemption against old rivals Leeds Rhinos.
"It's also fantastic to welcome Leigh Leopards into the competition and I'm confident they will help us deliver another brilliant season following a record-breaking year, with unprecedented participation numbers and viewing figures.
"We are also grateful for the continued support from Sky Sports. What a way to start the season, with the back-to-back defending champions taking on last year's most improved team - Wigan Warriors - who will no doubt be aiming for silverware this year."
Friday, May 16
Wigan Warriors vs York Valkyrie (5.30pm)
Saturday, May 17
St Helens Saints vs Leeds Rhinos (2pm)
Sunday, May 18
Warrington Wolves vs Huddersfield Giants
Leigh Leopards vs Burrow Raiders
Sunday, May 25
Leigh Leopards vs Warrington Wolves
York Valkyrie vs Leeds Rhinos (12pm)
Wigan Warriors vs Burrow Raiders (2pm)
Huddersfield Giants vs St Helens Saints (2pm)
Saturday, May 31
Leeds Rhinos vs Wigan Warriors (12pm)
Sunday, June 1
Warrington Wolves vs Burrow Raiders
York Valkyrie vs St Helens Saints (12pm)
Huddersfield Giants vs Leigh Leopards (2pm)
Saturday, June 14
Burrow Raiders vs St Helens Saints (2pm)
Sunday, June 15
Leeds Rhinos vs Warrington Wolves
Leigh Leopards vs York Valkyrie
Huddersfield Giants vs Wigan Warriors (2pm)
Saturday, June 21
St Helens Saints vs Warrington Wolves (2pm)
Sunday, June 22
Leeds Rhinos vs Barrow Raiders
Huddersfield Giants vs York Valkyrie (2pm)
Wigan Warriors vs Leigh Leopards (2pm)
Friday, June 27
Leeds Rhinos vs Leigh Leopards (5.15pm)
Sunday, June 29
Warrington Wolves vs York Valkyrie (12pm)
Burrow Raiders vs Huddersfield Giants (12pm)
Wigan Warriors vs St Helens Saints (2pm)
Friday, July 11
Leeds Rhinos vs St Helens Saints (5.30pm)
Sunday, July 13
Barrow Raiders vs Warrington Wolves (12pm)
York Valkyrie vs Leigh Leopards (12pm)
Wigan Warriors vs Huddersfield Giants (2pm)
Saturday, July 19
St Helens Saints vs Leigh Leopards (2pm)
Sunday, July 20
Leeds Rhinos vs Huddersfield Giants
York Valkyrie vs Barrow Raiders (12pm)
Warrington Wolves vs Wigan Warriors (12pm)
Sunday, July 27
York Valkyrie vs Huddersfield Giants (12pm)
Warrington Wolves vs St Helens Saints (12pm)
Barrow Raiders vs Leigh Leopards (2pm)
Wigan Warriors vs Leeds Rhinos (2pm)
Saturday, August 16
St Helens Saints vs Huddersfield Giants (2pm)
Sunday, August 17
Leigh Leopards vs Wigan Warriors
Barrow Raiders vs Leeds Rhinos (12pm)
York Valkyrie vs Warrington Wolves (12pm)
Saturday, August 23
St Helen Saints vs Barrow Raiders (2pm)
Sunday, August 24
Leigh Leopards vs Leeds Rhinos
York Valkyrie vs Wigan Warriors (12pm)
Huddersfield Giants vs Warrington Wolves (2pm)
Saturday, August 30
Barrow Raiders vs Wigan Warriors (2pm)
St Helens Saints vs York Valkyrie (2pm)
Sunday, August 31
Leigh Leopards vs Huddersfield Giants
Warrington Wolves vs Leeds Rhinos (12pm)
Friday, September 5
St Helens Saints vs Wigan Warriors (5.30pm)
Sunday, September 7
Warrington Wolves vs Leigh Leopards (12pm)
Barrow Raiders vs York Valkyrie (2pm)
Huddersfield Giants vs Leeds Rhinos (2pm)
Sunday, September 14
Leeds Rhinos vs York Valkyrie
Leigh Leopards vs St Helens Saints
Huddersfield Giants vs Barrow Raiders (2pm)
Wigan Warriors vs Warrington Wolves (2pm)